目的：探讨Numb 和VEGF 在原发性肝细胞癌（HCC）中的表达及其意义。 方法：免疫组化法检测60 例HCC 患者癌组织与癌旁组织，以及27 例肝外伤与肝血管瘤患者正常肝组 织中Numb 与VEGF 的表达，分析两者表达与HCC 患者临床病理因素以及预后的关系。 结果：与正常肝组织及癌旁组织比较，HCC 组织中Numb 阳性表达率明显降低，而VEGF 的阳性表 达率明显升高（均P<0.05），且在HCC 组织中两者表达呈负相关（r=-0.5248，P=0.01）；Numb 与VEGF 的表达均与患者TNM 分期、Edmondson 分级、门脉癌栓及包膜有无有关（均P<0.05）； Numb 阴性表达患者的总的生存期明显短于阳性表达者，而VEGF 情况则相反（均P<0.05）。 结论：在HCC 组织中，Numb 表达下调，而VEGF 表达上调，且两者之间的消长可能与HCC 的侵袭 性及不良预后密切相关。
Expressions of Numb and VEGF in hepatocellular carcinoma and the clinical significance
Objective: To investigate the expressions of Numb and vascular endothelial growth factor (VEGF) in primary hepatocellular carcinoma (HCC), and the clinical significance. Methods: The Numb and VEGF expressions in the surgical specimens of HCC tissue along with tumor adjacent tissues from 60 HCC patients, and normal liver tissues from 27 cases with liver injury or hepatic hemangioma were determined by immunohistochemical staining. The relations of Numb and VEGF expressions with the clinicopathologic factors and prognosis of the HCC patients were analyzed. Results: Compared with normal hepatic tissue and tumor adjacent tissue, the positive Numb expression rate was significantly decreased, while the positive VEGF expression was significantly increased in HCC tissue (both P<0.05), further, there was a negative relation between Numb and VEGF expression in HCC tissue (r=–0.5248, P=0.01). Both Numb and VEGF expressions were significantly associated with TNM stage, and Edmondson grade, and the presence or absence of portal cancer embolus and tumor capsule (all P<0.05). The overall survival period in patients with negative Numb expression was significantly shorter than in those with positive Numb expression, but the finding for VEGF expression was the opposite of that (both P<0.05). Conclusion: Numb expression is decreased and VEGF expression is increased in HCC tissue, and waxing and waning between them may closely relate to the invasiveness and poor prognosis of HCC.