目的：观察纳米金（GNP）人耐药肝癌细胞株耐药性的逆转作用。 方法：采用氯金酸柠檬酸三钠还原法制备并鉴定；分别用GNP 与阿霉素（ADM）组单独或联合作用于 ADM 耐药的肝癌细胞株HepG2/ADM，以未处理的HepG2/ADM 细胞为对照，用MTT 法检、流式细胞 术检测细胞的增殖与凋亡情况；紫外分光光度计检测HepG2/ADM 细胞经ADM 单独作用以及GNP 与 ADM 联合作用后细胞内ADM 浓度。 结果：与对照细胞比较，ADM 单独作用及GNP 与ADM 联合作用后，HepG2/ADM 的增殖均明显抑制、 凋亡率明显升高，但后者的作用明显强于前者（均P<0.05），而GNP 单独作用对细胞的增殖与凋亡无 明显影响（均P>0.05）；GNP+ADM 作用后，HepG2/ADM 细胞内的ADM 含量较ADM 单独作用后的 ADM 含量明显增加[（2.92±0.13）μg/L vs.（1.68±0.74）μg/L，P<0.05]。 结论：GNP 可增加HepG2/ADM 细胞对ADM 的敏感性，该作用可能与其增加HepG2/ADM 细胞内的 ADM 浓度有关。
Gold nanoparticles re-sensitizing adriamycin-resistant hepatocellular carcinoma cells: an experimental study
Objective: To investigate the drug resistance reversing effect of gold nanoparticles (GNPs) on drug-resistant human hepatocellular carcinoma cell line. Methods: GNPs were synthesized by reducing hydrogen tetrachloroaurate using tri-sodium citrate, and identified. Adriamycin (ADM)-resistant hepatocellular carcinoma HepG2/ADM cells were treated with GNP and adriamycin (ADM), alone or in combination, using HepG2/ADM cells without any treatment as a control, and then the cell proliferation and apoptosis were determined by MTT assay and flow cytometry, respectively. The intracellular ADM concentration of HepG2/ADM cells after exposure to ADM alone or GNP plus ADM were determined by ultraviolet-visible spectrophotometer. Results: Compared with control cells, the cell proliferation was inhibited and apoptosis rate was increased significantly in HepG2/ADM cells treated with ADM along or combination with GNP, and these effects were more remarkable in the latter than in the former (all P<0.05). The intracellular ADM concentration in HepG2/ADM cells exposed to GNP plus ADM was significantly higher than in those exposed to ADM alone [(2.92±0.13) μg/L vs. (1.68±0.74) μg/L, P<0.05]. Conclusion: GNP can enhance the sensitivity of HepG2/ADM cells to ADM, and this effect may be related to its increasing the intracellular ADM accumulation in HepG2/ADM cells.