基础研究(Basic Research)

纳米金增加阿霉素耐药肝癌细胞株敏感性的实验研究

Published at: 2015年第24卷第1期

邵明涛 1 , 潘运龙 1,2 , 覃莉 3 , 丁晖 1 , 赵晓旭 2 , 吴晓波 1
1 暨南大学附属第一医院 胃肠外科,广东 广州 510632
2 暨南大学附属第一医院 伽玛刀中心,广东 广州 510632
3 暨南大学医学院 组织胚胎学教研室,广东 广州 510632
通讯作者 运龙 潘 Email: tpanyl@jnu.edu.cn
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.01.010
基金:
国家基础研究973 计划资助项目 2010CB833603
国家自然科学基金资助项目 81472849
暨南大学第一临 床医学院科研培育基金资助项目 511005004
暨南大学第一临床医学院开放基金资助项目 511005026

摘要

目的:观察纳米金(GNP)人耐药肝癌细胞株耐药性的逆转作用。 方法:采用氯金酸柠檬酸三钠还原法制备并鉴定;分别用GNP 与阿霉素(ADM)组单独或联合作用于 ADM 耐药的肝癌细胞株HepG2/ADM,以未处理的HepG2/ADM 细胞为对照,用MTT 法检、流式细胞 术检测细胞的增殖与凋亡情况;紫外分光光度计检测HepG2/ADM 细胞经ADM 单独作用以及GNP 与 ADM 联合作用后细胞内ADM 浓度。 结果:与对照细胞比较,ADM 单独作用及GNP 与ADM 联合作用后,HepG2/ADM 的增殖均明显抑制、 凋亡率明显升高,但后者的作用明显强于前者(均P<0.05),而GNP 单独作用对细胞的增殖与凋亡无 明显影响(均P>0.05);GNP+ADM 作用后,HepG2/ADM 细胞内的ADM 含量较ADM 单独作用后的 ADM 含量明显增加[(2.92±0.13)μg/L vs.(1.68±0.74)μg/L,P<0.05]。 结论:GNP 可增加HepG2/ADM 细胞对ADM 的敏感性,该作用可能与其增加HepG2/ADM 细胞内的 ADM 浓度有关。


Gold nanoparticles re-sensitizing adriamycin-resistant hepatocellular carcinoma cells: an experimental study

Abstract

Objective: To investigate the drug resistance reversing effect of gold nanoparticles (GNPs) on drug-resistant human hepatocellular carcinoma cell line. Methods: GNPs were synthesized by reducing hydrogen tetrachloroaurate using tri-sodium citrate, and identified. Adriamycin (ADM)-resistant hepatocellular carcinoma HepG2/ADM cells were treated with GNP and adriamycin (ADM), alone or in combination, using HepG2/ADM cells without any treatment as a control, and then the cell proliferation and apoptosis were determined by MTT assay and flow cytometry, respectively. The intracellular ADM concentration of HepG2/ADM cells after exposure to ADM alone or GNP plus ADM were determined by ultraviolet-visible spectrophotometer. Results: Compared with control cells, the cell proliferation was inhibited and apoptosis rate was increased significantly in HepG2/ADM cells treated with ADM along or combination with GNP, and these effects were more remarkable in the latter than in the former (all P<0.05). The intracellular ADM concentration in HepG2/ADM cells exposed to GNP plus ADM was significantly higher than in those exposed to ADM alone [(2.92±0.13) μg/L vs. (1.68±0.74) μg/L, P<0.05]. Conclusion: GNP can enhance the sensitivity of HepG2/ADM cells to ADM, and this effect may be related to its increasing the intracellular ADM accumulation in HepG2/ADM cells.


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引用

引用本文: 明涛 邵, 运龙 潘, 莉 覃, 晖 丁, 晓旭 赵, 晓波 吴. 纳米金增加阿霉素耐药肝癌细胞株敏感性的实验研究[J]. 中国普通外科杂志, 2015, 24(1): 52–56.
Cite this article as: SHAO Mingtao, PAN Yunlong, QIN Li, DING Hui, ZHAO Xiaoxu, WU Xiaobo . Gold nanoparticles re-sensitizing adriamycin-resistant hepatocellular carcinoma cells: an experimental study [J]. Chin J Gen Surg, 2015, 24(1): 52–56.