目的：探讨结直肠癌肝转移（CRLM）组织中TGF-β1、Smad7 表达和树突状细胞（DC）的变化及临床意义。 方法：采用免疫组化法检测TGF-β1、Smad7、CD1α（未成熟DC 标志）和CD83（成熟DC 标志） 在CRLM 组织和CRLM 术后切缘阴性的正常肝组织中的表达，分析CRLM 组织中以上指标表达之间的 关系以及与原发灶特征的关系。 结果：CRLM 组织中TGF-β1、Smad7、和CD1α 的表达明显高于正常肝组织组（均P<0.05）， 且 CRLM 组织中彼此表达之间呈正相关关系（r=0.455、r=0.623、r=0.448，均P<0.05）；CD83 在CRLM 组织中基本为全阴性表达，而在正常肝组织组中仅2 例呈弱阳性表达。TGF-β1、Smad7、和CD1α 的表达均与CRLM 患者原发灶的肿瘤浸润深度、有无淋巴结转移有关（均P<0.05），而与原发灶肿瘤 分化程度无关（P>0.05）。 结论：TGF-β1、Smad7α 的高表达及未成熟DC 数量的增加可能共同促进CRLM 的发生、发展。
Preliminary study of roles of TGF- 1, Smad7 and dendritic cells in colorectal cancer liver metastases
Objective: To investigate the expressions of TGF-β1 and Smad7 and change of dendritic cells (DCs) in colorectal cancer liver metastases (CRLM) tissue and the clinical significance. Methods: The expressions of TGF-β1, Smad7, CD1α (marker of immature DCs) and CD83 (marker of mature DCs) in resection specimens of CRLM tissue and normal liver tissue around the CRLM with negative margin were determined by immunohistochemical staining. The mutual relations among these expressions in CRLM tissue and their relations with the characteristics of the primary tumor were analyzed. Results: The expressions of TGF-β1, Smad7, and CD1α in CRLM tissue were significantly higher than those in normal liver tissue (all P<0.05), and their expressions showed mutual positive correlations in CRLM tissue (r=0.455, r=0.623, r=0.448, all P<0.05); CD83 expression was negative in almost all the studied CRLM tissue specimens, and only two cases of weak positive expression were found in the normal liver tissue specimens. All expressions of TGF-β1, Smad7 and CD1α were significantly related to the depth of invasion of the primary tumor and lymph node metastasis (all P<0.05), but irrelevant to differentiation of the primary tumor (P>0.05) in CRLM patients. Conclusion: The high TGF-β1 and Smad7 expressions and increased immature DCs number may together promote the occurrence and development of CRLM.