目的：探讨转化生长因子β 激活酶1（TAK1）在结肠癌组中的表达与临床意义。方法：收集141 例结肠癌患者手术标本，用免疫组织化学方法检测TAK1 蛋白在结肠癌及癌旁正常组织中的表达，并分析其表达与患者临床病理因素及预后的关系，同时检测结肠癌组织中K-ras 基因突变情况，分析TAK1 表达与K-ras 基因突变的关系。结果：TAK1 在结肠癌组织中的阳性表达率明显高于癌旁正常组织（68.8% vs. 16.3%，P<0.05）；TAK1 的阳性表达与Dukes 分期、肿瘤分化程度和淋巴结转移有关（P<0.05）；TAK1 阳性表达患者的5 年生存率明显低于低表达的患者（P<0.05）；TAK1 阳性表达的结肠癌组织K-ras 基因的突变率明显高于TAK1 阴性表达的结肠癌组织（52.6% vs. 13.6%，P<0.05）。结论：TAK1 可能参与了结肠癌的恶性进展，且TAK1 的表达可能与K-ras 基因突变密切相关。
Expression and clinical significance of transforming growth factor -activated kinase 1 in colon cancer
Objective: To investigate the expression of transforming growth factor β-activated kinase 1 (TAK1) in colon cancer and the clinical significance. Methods: Surgical specimens from 141 patients with colon cancer were collected. The TAK1 expression in colon cancer tissues and their adjacent normal tissues were determined by immunohistochemical staining, and the relations of TAK1 expression with clinicopathologic factors as well as prognosis of the patients were analyzed. Meanwhile, the K-ras gene mutation in the colon cancer tissues was detected, and the relationship between TAK1 expression and K-ras gene mutation was examined. Results: The positive expression rate of TAK1 in colon cancer tissues was significantly higher than that in adjacent normal tissues (68.8% vs. 16.3%, P<0.05). The positive TAK1 expression was significantly associated with Dukes stage, degree of tumor differentiation and lymph node metastasis (P<0.05), and the 5-year survival rate in patients with TAK1 positive tumor was significantly lower than in those with its low expression(P<0.05). The incidence of K-ras gene mutation in TAK1 positive colon cancer tissues was significantly higher than that in those with TAK1 negative expression (52.6% vs. 13.6%, P<0.05). Conclusion: TAK1 may be involved in malignant progression of colon cancer, and its expression is probably related to K-ras gene mutation.