目的：探讨乳腺癌组织中Skp2 与p27Kip1 的表达及其意义。方法：采用免疫组化法两步检测法检测正常乳腺组织、导管上皮不典型增生组织（ADH）以及浸润性导管癌（IDC）组织中的Skp2 与p27Kip1 的表达，并分析两者表达与乳腺癌淋巴结转移及组织学分级的关系。结果：Skp2 阳性表达率在正常乳腺组织、ADH 组织、IDC 组织依次升高，伴腋窝淋巴结转移的IDC组织中Skp2 阳性表达率明显高于无淋巴结转移的IDC 组织，而p27Kip1 的表达则与Skp2 的表达呈反向变化（均P<0.05）；不同组织学分级IDC 组织间Skp2 阳性率、p27Kip1 阳性率差异均有统计学意义（均P<0.05），且Skp2 阳性表达与IDC 组织学分级呈正相关（r=0.492，P<0.05），而p27Kip1 的阳性表达与IDC 组织学分级呈负相关（r=-0.327，P<0.05）。结论：乳腺癌组织中Skp2 表达上调而p27Kip1 表达下调，两者的变化程度与乳腺癌的恶性生物学特征密切相关。
Relations of Skp2 and p27 Kip1 expressions with clinicopathologic features of breast cancer
Objective: To investigate the Skp2 and p27 Kip1 expressions in breast cancer tissue and the significance. Methods: The Skp2 and p27Kip1 expressions in normal breast tissues, atypical ductal hyperplasia (ADH) tissue and invasive ductal carcinoma (IDC) tissue were determined by immunohistochemical staining. The relations of their expressions with lymph node metastasis and histologic grade of breast cancer were also analyzed. Results: The Skp2 positive expression rate was increased in ascending order in normal breast tissue, ADH tissue and IDC tissue, which was significantly higher in IDC tissue with axillary lymph node metastasis than that in IDC tissue without axillary lymph node metastasis, while the opposite was true for p27Kip1 positive expression rate (all P<0.05). Both Skp2 and p27Kip1 positive expression rates were significantly different among IDC tissues with different histologic grades (both P<0.05), moreover, Skp2 positive expression had a positive correlation with histologic grade of IDC (r=0.492, P<0.05), while p27Kip1 positive expression had a negative correlation with histologic grade of IDC (r=–0.327, P<0.05). Conclusion: Skp2 expression is up-regulated and p27Kip1 expression is down-regulated in breast cancer tissue, and their changing degrees are closely related to the malignant biological behaviors of breast cancer.