目的：探讨乳腺癌患者CD4+CD25+Foxp3+ 调节性T 细胞（简称Foxp3+Treg）的变化及意义。方法：选择40 例乳腺癌患者和32 例乳腺良性肿瘤患者，采用流式细胞术检测外周血Foxp3+Treg、CD8+CD28+T 细胞、NK 细胞水平；用Western blot 和RT-PCR 病变乳腺组织Foxp3 蛋白与mRNA 表达。结果：乳腺癌患者外周血中Foxp3+Treg 比例较乳腺良性肿瘤患者明显升高，而CD8+CD28+T 细胞、NK细胞比例明显降低（均P<0.05），且乳腺癌患者外周血Foxp3+Treg 水平与CD8+CD28+T 细胞和NK 细胞水平呈负相关（r=-0.631，r=-0.578，均P<0.05）；乳腺癌患者术后外周血Foxp3+Treg 水平较术前明显降低（P<0.05）。乳腺癌组织中Foxp3 蛋白与mRNA 的表达均较乳腺良性肿瘤组织明显升高（均P<0.05）。结论：Foxp3+Treg 和其标记分子Foxp3 在乳腺癌患者中的表达增加，且可能通过抑制CD8+CD28+T 细胞和NK 细胞而产生肿瘤免疫抑制。
Alteration of CD4+CD25+Foxp3+ regulartory T cells in breast cancer patients and its significance
Objective: To investigate the alteration of CD4+CD25+Foxp3+ regulartory T cells (hereinafter abbreviated as Foxp3+ Tregs) in breast cancer patients and its significance. Methods: Forty patients with breast cancer and 32 patients with benign breast tumors were enrolled. The levels of Foxp3+ Tregs, CD8+CD28+ T cells and NK cells in peripheral blood of the patients were measured by flow cytometry, and the Foxp3 protein and mRNA expressions in breast lesions were determined by Western blot and RT-PCR, respectively. Results: The ratio of Foxp3+ Tregs was significantly higher, while the ratios of both CD8+CD28+ T cells and NK cells in peripheral blood were significantly lower in breast cancer patients than those in patients with benign breast tumor (all P<0.05), and there was a negative correlation between the level of Foxp3+ Tregs and either the level of CD8+CD28+ T cells or NK cells in peripheral blood of breast cancer patients (r=–0.631, r=–0.578, both P<0.05); the peripheral blood level of Foxp3+ Tregs in breast cancer patients was significantly decreased after operation compared with the level before operation (P<0.05). Either protein or mRNA level of Foxp3 in breast cancer tissue was significantly higher than that in benign breast tumor tissue (both P<0.05). Conclusion: Foxp3+ Treg and its molecular marker Foxp3 are increased in breast cancer patients, which may probably contribute to the tumor immunosuppression through inhibition of CD8+CD28+ T and NK cells.