基础研究(Basic Research)

microRNA-25 高表达与直肠癌细胞的侵袭和迁移的关系

Published at: 2015年第24卷第12期

徐晓慧 1 , 孙欣 1 , 刘娜 1 , 矫健 1 , 李秋阳 1 , 刘京平 1 , 林方才 1
1 首都医科大学电力教学医院 普通外科,北京 100073
通讯作者 方才 林 Email: fc-lin@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.12.015
基金:

摘要

目的:探讨microRNA-25(miR-25)在直肠癌细胞中的表达及作用。 方法:检测多种不同直肠癌细胞中miR-25 的表达,并检测直肠癌细胞转染miR-25 前体(pre-miR-25) 上调miR-25 的表达或转染miR-25 抑制剂(anti-miR-25)下调miR-25 的表达后生物学行为的变化。 结果:与正常直肠黏膜组织比较,不同的直肠癌细胞中miR-25 的表达均不同程度的明显升高(均 P<0.05)。在miR-25 表达水平相对较低的直肠癌HR-834 细胞中转染pre-miR-25, 在miR-25 高表 达的直肠癌SW-837 细胞中转染anti-miR-25 后, 两种细胞的增殖、细胞周期、凋亡无明改变( 均 P>0.05),但侵袭及迁移能力在HR-834 细胞的明显增强,SW-837 细胞减弱(均P<0.05)。 结论:miR-25 在直肠癌细胞中的表达升高,且其升高程度与细胞的侵袭和迁移能力密切相关。


Relations of high microRNA-25 expression with migration and invasion of rectal carcinoma cells

Abstract

Objective: To investigate microRNA-25 (miR-25) expression in rectal carcinoma cells and its action. Methods: The miR-25 expressions in several different rectal cell lines were determined, and then the changes in biological behaviors in rectal cancer cells after up- or down-regulation of miR-25 expression with miR-25 precursor (pre-miR-25) or miR-25 inhibitor (anti-miR-25) transfection were examined. Results: Compared with normal rectal mucosal tissue, the miR-25 expressions in all tested rectal cancer cells were significantly increased with varying degrees (all P<0.05). In rectal cancer HR-834 cells with relatively low miR-25 expression after pre-miR-25 transfection and in rectal cancer SW-837 cells with high miR-25 expression after antimiR- 25 transfection, the proliferation, cell cycle and apoptosis showed no significant change (all P<0.05), but the invasion and migration abilities in HR-834 cells were increased, and SW-837 cells were decreased significantly (all P<0.05). Conclusion: The miR-25 expression is elevated in rectal cancer cells, and its degree of increase is closely related to the invasion and migration abilities of rectal cancer cells.


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引用

引用本文: 晓慧 徐, 欣 孙, 娜 刘, 健 矫, 秋阳 李, 京平 刘, 方才 林. microRNA-25 高表达与直肠癌细胞的侵袭和迁移的关系[J]. 中国普通外科杂志, 2015, 24(12): 1716-1721.
Cite this article as: XU Xiaohui, SUN Xin, LIU Na, JIAO Jian, LI Qiuyang, LIU Jingping, LIN Fangcai . Relations of high microRNA-25 expression with migration and invasion of rectal carcinoma cells[J]. Chin J Gen Surg, 2015, 24(12): 1716-1721.