基础研究(Basic Research)

奥曲肽诱导人结肠癌细胞凋亡与细胞周期的影响及机制

Published at: 2015年第24卷第4期

龙建武 1 , 梁庆模 2 , 周筱筠 1 , 李晶 1 , 刘龙飞 1 , 肖帅 1 , 卢先州 1
1 南华大学附属南华医院 普通外科,湖南 衡阳 421002
2 南华大学附属南华医院 肿瘤外科,湖南 衡阳 421002
通讯作者 先州 卢 Email: 158452871@qq.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.04.014
基金:
湖南省自然科学基金省市联合资助项目 12JJ9030

摘要

目的:探讨奥曲肽诱导人结肠癌细胞凋亡与细胞周期的影响及机制。 方法:奥曲肽、GSK-3β 抑制剂LiCl 单独或联合作用于人结肠癌SW480 细胞后,用流式细胞术检测 SW480 细胞的凋亡与细胞周期,以及用DNA 凝胶电泳实验进一步验证细胞凋亡;Western blot 检测 SW480 细胞中GSK-3β、p-GSK3-β(Tyr216)及p-GSK-3β(Ser9)蛋白表达。 结果:与未处理的空白对照组SW480 比较,奥曲肽处理SW480 细胞后,细胞凋亡率与G0/G1 细胞明显 升高,凝胶电泳出现典型的“梯形”DNA 条带,总GSK-3β 蛋白与p-GSK3β(Tyr216)表达明显上调, 而p-GSK3β(Ser9)蛋白表达明显下降(均P<0.05);LiCl 单独作用对SW480 细胞的凋亡与细胞周 期无明显影响(均P>0.05),但与奥曲肽联合作用能明显削弱奥曲肽对SW480 的促凋亡与细胞周期阻 滞作用(均P<0.05)。 结论:奥曲肽能有效诱导SW480 细胞凋亡与细胞周期阻滞,该作用可能与其上调GSK-3β 蛋白的表达, 并调节GSK-3β 蛋白的磷酸化水平有关。


Influence of octreotide on apoptosis and cell cycle in human colon cancer cells and the mechanism

Abstract

Objective: To investigate the influence of octreotide on apoptosis and cell cycle in human colon cancer cells and the mechanism. Methods: Human colon cancer SW480 cells were exposed to octreotide or GSK-3β inhibitor LiCl alone or in combination; the apoptosis and cell cycle in SW480 were analyzed by flow cytometry, and the apoptosis was also verified by DNA agarose gel electrophoresis. In addition, the expressions of GSK-3β, p-GSK3β (Tyr216) and p-GSK3β (Ser9) in SW480 cells were determined by Western blot analysis. Results: Compared with the blank control group of untreated SW480 cells, in SW480 cells after octreotide treatment, the apoptosis and ratio of G0/G1 phase cells were significantly increased, typical DNA ladders were present in the agarose gel electrophoresis, and the total GSK-3β protein and p-GSK3β (Tyr216) protein expressions were significantly increased, while the p-GSK3β (Ser9) protein expression was significantly decreased (all P<0.05); LiCl treatment alone exerted no influence on apoptosis and cell cycle in SW480 cells (both P>0.05), but it significantly weakened the apoptosis-inducing and cell cycle arrest effect of octreotide in SW480 cells in combination with octreotide (both P<0.05). Conclusion: Octreotide can effectively induce apoptosis and cell cycle arrest in SW480 cells, which may be related to its increasing GSK-3β protein expression and regulating phosphorylation level of GSK-3β.


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引用

引用本文: 建武 龙, 庆模 梁, 筱筠 周, 晶 李, 龙飞 刘, 帅 肖, 先州 卢. 奥曲肽诱导人结肠癌细胞凋亡与细胞周期的影响及机制[J]. 中国普通外科杂志, 2015, 24(4): 532-537.
Cite this article as: LONG Jianwu, LIANG Qingmo, ZHOU Xiaojun, LI JING, LIU Longfei, XIAO Shuai, LU Xianzhou . Influence of octreotide on apoptosis and cell cycle in human colon cancer cells and the mechanism[J]. Chin J Gen Surg, 2015, 24(4): 532-537.