基础研究(Basic Research)

抑制过氧化物酶1表达对肝癌细胞放射敏感性的影响

Published at: 2016年第25卷第2期

涂青松 1 , 何剪太 2 , 陈伟 2
1 中南大学湘雅医院肿瘤放疗科,湖南 长沙 410008
2 中南大学湘雅医院肝胆肠研究中心 ,湖南 长沙 410008
通讯作者 伟 陈 Email: chenwei_cs@aliyun.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.02.015
基金:

摘要

目的:探讨抑制过氧化物酶1(Prx-1)对肝癌细胞放射敏感性的影响。方法:选用人肝癌HepG2细胞,用分次放疗放射剂量递增法诱导放射抵抗的肝癌细胞(RR-HepG2),检测RR-HepG2细胞与亲代HepG2细胞中Prx-1的表达,以及两种细胞在接受相同放射处理后存活率与迁移、侵袭能力。用表达shRNA-Prx-1的质粒转染两种细胞后,再次检测上述指标的变化。结果:与亲代HepG2细胞比较,RR-HepG2细胞Prx-1的mRNA与蛋白表达明显增高;放射处理后,RR-HepG2细胞的存活率升高,迁移能力及侵袭能力增强(均P<0.05)。转染shRNA-Prx-1后,与各自转染阴性对照序列的细胞比较,两种细胞Prx-1的mRNA与蛋白表达均明显降低;接受放射处理后,两种细胞的存活率均降低,迁移能力以及侵袭能力均明显减弱(均P<0.05)。结论:肝癌细胞对放射的敏感性降低可能与Prx-1的表达有关,调控Prx-1的表达可望成为增强肝癌细胞放射敏感性的有效途径。


Enhancement of radiosensitivity of hepatocellular carcinoma cells by inhibition of peroxiredoxin 1 expression

Abstract

Objective: To investigate the effect of peroxiredoxin 1 (Prx-1) inhibition on radiosensitivity of hepatocellular carcinoma (HCC) cells. Methods: Using human HCC HepG2 cells, the radioresistant HCC cells (RR-HepG2) were induced by fractionated irradiation with ascending radiation dose levels. In RR-HepG2 cells and their parental HepG2 cells, the Prx-1 expression was examined, and after undergoing the same radiation treatment, the survival rate and the invasion and migration abilities were determined. Then, in these two types of cells after transfection with the plasmids bearing shRNA-Prx-1, the above parameters were determined again. Results: In RR-HepG2 cells compared with their parental HepG2 cells, both Prx-1 mRNA and protein expressions were significantly increased; the survival rate, and invasion and migration abilities were significantly increased after radiation treatment (all P<0.05). After shRNA-Prx-1 transfection, in both types of cells compared with their counterparts transfected with negative control sequences, both Prx-1 mRNA and protein expressions were significantly decreased; the survival rate, and invasion and migration abilities were significantly decreased after radiation treatment (all P<0.05). Conclusion: The decreased radiosensitivity in HCC cells may probably be associated with Prx-1 expression, and regulating its expression could potentially be an effective approach for enhancing radiosensitivity of HCC cells.


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引用

引用本文: 青松 涂, 剪太 何, 伟 陈. 抑制过氧化物酶1表达对肝癌细胞放射敏感性的影响[J]. 中国普通外科杂志, 2016, 25(2): 245-251.
Cite this article as: TU Qingsong, HE Jiantai, CHEN Wei . Enhancement of radiosensitivity of hepatocellular carcinoma cells by inhibition of peroxiredoxin 1 expression[J]. Chin J Gen Surg, 2016, 25(2): 245-251.