基础研究(Basic Research)

乳腺癌组织中miR-34a与VEGF的关系及临床意义

Published at: 2016年第25卷第5期

迟婷 1 , 姜晓燕 1
1 甘肃省肿瘤医院 乳腺外科,甘肃 兰州 730050
通讯作者 晓燕 姜 Email: 627672845@qq.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.05.010
基金:

摘要

目的:探讨乳腺癌组织中miR-34a与VEGF的关系及临床意义。方法:检测40对乳腺癌及其癌旁组织组织中miR-34a与VEGF的表达,分析乳腺癌中miR-34a表达与临床病理因素及VEGF表达的关系;用双荧光素酶报告系统检测miR-34a对乳腺癌细胞MCF-7中VEGF转录活性的影响;用miR-34a模拟物转染MCF-7细胞后,检测细胞增殖情况与VEGF及下游Akt蛋白表达的变化。结果:与癌旁组织比较,乳腺癌组织中miR-34a表达明显降低,而VEGF表达明显升高(均P<0.05);乳腺癌中miR-34a的表达与TNM分期有关(P<0.05),且与VEGF的表达呈负相关(r2=0.4469,P=0.0033)。miR-34a模拟物转染后,MCF-7细胞中VEGF的转录活性明显抑制;增殖率明显降低,VEGF的表达以及Akt的磷酸化水平明显下调(均P<0.05)。结论:miR-34a在乳腺癌组织中表达降低,削弱了对VEGF及其下游Akt磷酸化的抑制,从而促进了乳腺癌的生长。


Relationship between miR-34a and VEGF expressions in breast cancer tissue and the clinical significance

Abstract

Objective: To investigate the relationship between miR-34a and VEGF expressions in breast cancer and the clinical significance. Methods: The miR-34a and VEGF expression in 40 paired specimens of breast cancer and adjacent tissue were detected, and the relations of miR-34a expression with clinicopathologic factors and VEGF expression in breast cancer were analyzed. The influence of miR-34a on transcriptional activity of VEGF in breast cancer MCF-7 cells was determined by dual-luciferase reporter assay system. In MCF-7 cells after transfection with miR-34a mimics, the changes in cell proliferation and expression of VEGF and its down-stream Akt protein were determined. Results: In breast cancer tissue compared with adjacent tissue, the miR-34a expression was decreased while VEGF expression was increased significantly (both P<0.05), and the miR-34a expression was significantly related to TNM stage of breast cancer tissue (P<0.05) and was negatively correlated to VEGF expression in breast cancer tissue (r2=0.4469, P=0.0033). After miR-34a mimics transfection in MCF-7 cells, the transcriptional activity of VEGF was inhibited, proliferation rate was reduced, and the VEGF expression and phosphorylation level of Akt protein was down-regulated significantly (all P<0.05). Conclusion: MiR-34a expression in breast cancer tissue is decreased, which reduces the inhibition on VEGF and its down-stream Akt phosphorylation, and thereby facilitate the growth of breast cancer.


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引用

引用本文: 婷 迟, 晓燕 姜. 乳腺癌组织中miR-34a与VEGF的关系及临床意义[J]. 中国普通外科杂志, 2016, 25(5): 680-685.
Cite this article as: CHI Ting, JIANG Xiaoyan . Relationship between miR-34a and VEGF expressions in breast cancer tissue and the clinical significance[J]. Chin J Gen Surg, 2016, 25(5): 680-685.