基础研究(Basic Research)

Tspan8过表达与肝癌转移潜能及预后的关系

Published at: 2016年第25卷第7期

王春玲 1 , 陈良 1 , 周开伦 1 , 张震生 1 , 唐荣 1 , 陈平平 1 , 许达峰 1 , 陈骋 1 , 李灼日 1 , 武金才 1
1 海南省人民医院 肝胆胰、器官移植外科,海南 海口 570311
通讯作者 金才 武 Email: wujincai021@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.07.011
基金:
国家自然科学基金资助项目 81160310;81260367
海南省自然科学基金资助项目 812155;814318

摘要

目的:探讨Tspan8表达与肝癌侵袭转移及预后之间关系。方法:用qRT-PCR和Western blot检测不同肝癌细胞系及80例肝癌患者手术标本中Tspan8的mRNA与蛋白表达;应用组织芯片检测352例肝癌组织样本中Tspan8的表达,分析Tspan8表达与肝癌临床病理因素及复发与预后的关系。结果:Tspan8的mRNA与蛋白表达在高转移潜能肝癌细胞系(MHCC97-H、MHCC97-L)中的表达明显高于低转移潜能的肝癌细胞系(PLC/PRF/5、SMMC7721、HepG2),在肝癌组织中的表达明显高于癌旁与正常肝组织,且在有肝内转移或血管侵犯患者癌组织中的表达明显高于无肝内转移及血管侵犯患者的癌组织(均P<0.05)。Tspan8高表达是肝癌术后复发转移(HR=1.64,95% CI=1.21~2.23,P=0.002)和术后生存(HR=1.66,95% CI=1.23~2.25,P=0.001)的独立危险因素,Tspan8高表达患者术后5年总生存率明显低于Tspan8低表达组,术后复发时间明显短于低表达患者(均P<0.05)。结论:Tspan8促进肝癌侵袭转移,Tspan8高表达患者预后不良。


Association of Tspan8 over-expression with metastastic potential and prognosis of hepatocellular carcinoma

Abstract

Objective: To investigate the association of Tspan8 expression with metastatic potential and prognosis in hepatocellular carcinoma (HCC). Methods: The mRNA and protein expressions in different HCC cell lines and surgical specimens of 80 HCC patients were determined by qRT-PCR and Western blot analysis, and Tspan8 expressions in tissue samples from another 352 HCC patients were detected by tissue microarray. The relations of Tspan8 expression with clinicopathologic factors as well as recurrence and prognosis in HCC patients were analyzed. Results: Both mRNA and protein expressions of Tspan8 were significantly higher in HCC cell lines with high metastatic potential (MHCC97-H, MHCC97-L) than those in HCC cell lines with low metastatic potential (PLC/PRF/5, SMMC7721, HepG2), significantly higher in HCC tissue than those in tumor-adjacent tissue or normal liver tissue, and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion (all P<0.05). High Tspan8 expression was independent risk factor for postoperative recurrence (HR=1.64, 95% CI=1.21–2.23, P=0.002) and survival (HR=1.66, 95% CI=1.23–2.25, P=0.001), and in patients with high Tspan8 expression compared with those with low Tspan8 expression, the postoperative 5-year overall survival was significantly decreased and time to tumor recurrence was significantly shortened (both P<0.05). Conclusion: Tspan8 can promote HCC metastasis and invasion, and patients with high Tspan8 may face a poor prognosis.


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引用

引用本文: 春玲 王, 良 陈, 开伦 周, 震生 张, 荣 唐, 平平 陈, 达峰 许, 骋 陈, 灼日 李, 金才 武. Tspan8过表达与肝癌转移潜能及预后的关系[J]. 中国普通外科杂志, 2016, 25(7): 998-1004.
Cite this article as: WANG Chunling, CHEN Liang, ZHOU Kailun, ZHANG Zhensheng, TANG Rong, CHEN Pingping, XU Dafeng, CHEN Cheng, LI Zhuori, WU Jincai . Association of Tspan8 over-expression with metastastic potential and prognosis of hepatocellular carcinoma[J]. Chin J Gen Surg, 2016, 25(7): 998-1004.