基础研究(Basic Research)

抑癌基因Merlin与干细胞标记物Nanog在肝细胞癌中表达的关系及意义

Published at: 2016年第25卷第7期

欧阳锡武 1 , 冯铁诚 1 , 张赛 2 , 陶一明 1 , 王志明 1
1 中南大学湘雅医院肝脏外科,湖南 长沙410008
2 中南大学湘雅医院医学科学研究中心,湖南 长沙410008
通讯作者 志明 王 Email: wzmxycsu@hotmail.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.07.013
基金:
国家自然科学基金面上资助项目 81372631
中南大学湘雅医院2014 年度临床科研基金资助项目 2014L07
湖南省发改委科研基金资助项目 湘发改高技[2013]1199
“湘雅临床大数据系统”资助建设项目 22

摘要

目的:探讨肝细胞癌(HCC)抑癌基因失活与肝癌干细胞(LCSC)的关系及其临床意义。方法:用免疫组化法检测116例HCC组织标本中抑癌基因Merlin与LCSC表面标记物Nanog的蛋白表达,分析两者的表达与HCC患者临床病理因素及预后间的关系。结果:按X-tile软件计算得出的Merlin与Nanog表达量的分界值,116例HCC中Merlin低表达者92例(79.3%),高表达者24例(20.7%);Nanog高表达者36例(31.0%),低表达者80例(69.0%)。单因素分析显示,分化较差,有卫星灶、静脉浸润、出血坏死的HCC组织中Merlin低表达,而Nanog高表达(均P<0.05);多因素分析显示,Merlin低表达和Nanog高表达是HCC术后存活和复发的独立的预测指标(均P<0.05)。将患者分为低危组(Merlin高表达并Nanog低表达)、中危组(Merlin高表达并Nanog高表达或Merlin低表达并Nanog低表达)、高危组(Merlin低表达并Nanog高表达)分析,3组患者的术后总体生存率(低危组>中危组>高危组)和复发率(低危组<中危组<高危组)差异均有统计学意义(均P<0.05)。结论:抑癌基因Merlin失活与Nanog高表达有一定关系,两者的消长与HCC患者预后密切相关。


Relationship between expressions of cancer suppressor gene Merlin and stem cell marker Nanog in hepatocellular carcinoma and the significance

Abstract

Objective: To investigate the relationship between inactivation of cancer suppressor gene and liver cancer stem cells (LCSC) in hepatocellular carcinoma (HCC) and the clinical significance. Methods: The protein expressions of cancer suppressor gene Merlin and LCSC surface marker in 116 specimens of HCC tissue were determined by immunohistochemical staining, and then the relations of their expressions with clinicopathologic factors and prognosis of HCC patients were analyzed. Results: According to the cut-off values of Merlin and Nanog expression levels calculated by X-tile software, in the 116 HCC patients, 92 cases (79.3%) had low Merlin expression and 24 cases (20.7%) had high Merlin expression; 36 cases (31.0%) had high Nanog expression and 80 cases (69.0%) had low Nanog expression. Univariate analysis showed that HCC tissues with poor differentiation, satellite lesions, venous invasion or hemorrhagic necrosis had low Merlin but high Nanog expression (all P<0.05). Multivariate analysis showed that low Merlin expression and high Nanog expression were independent predictors for postoperative survival and recurrence (both P<0.05). After the patients were divided into low risk group (high Merlin and low Nanog expression), medial risk group (high Merlin and high Nanog expression or low Merlin and low Nanog expression) and high risk group (low Merlin and high Nanog expression), the analysis demonstrated that both overall survival rate (low risk group>medial risk group>high risk group) and recurrence rate (low risk group


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引用

引用本文: 锡武 欧阳, 铁诚 冯, 赛 张, 一明 陶, 志明 王. 抑癌基因Merlin与干细胞标记物Nanog在肝细胞癌中表达的关系及意义[J]. 中国普通外科杂志, 2016, 25(7): 1011-1016.
Cite this article as: OUYANG Xiwu, FENG Tiecheng, ZHANG Sai, TAO Yiming, WANG Zhiming . Relationship between expressions of cancer suppressor gene Merlin and stem cell marker Nanog in hepatocellular carcinoma and the significance [J]. Chin J Gen Surg, 2016, 25(7): 1011-1016.