基础研究(Basic Research)

HSF-1在HMGB1诱导的炎症反应中的作用

Published at: 2016年第25卷第9期

尹朝奇 1 , 贺全勇 1 , 罗成群 1 , 周建大 1 , 陈佳 1 , 李萍 1 , 朱颉 1 , 彭浩 1 , 徐阳成 1 , 陶如意 1
1 中南大学湘雅三医院 烧伤整形科,湖南 长沙 410013
通讯作者 朝奇 尹 Email: yinchaoqi@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.09.011
基金:
湖南省卫生厅科研基金资助项目 B2012-030

摘要

目的:探讨热休克转录因子1(HSF-1)在高迁移率族蛋白1(HMGB1)所诱导的炎症反应中的作用。方法:不同浓度HMGB1作用于RAW264.7细胞不同时间后,用ELISA法检测细胞上清液TNF-α水平;分别用免疫荧光法与Western blot法检测HMGB1作用后,RAW264.7细胞NF-κB核转移情况与HSF-1表达。观察干扰HSF-1表达后,HMGB1诱导RAW264.7细胞TNF-α表达的变化。结果:HMGB1作用后,RAW264.7细胞分别在4、12 h出现两次TNF-α分泌高峰,且TNF-α的释放量随HMGB1浓度的增加而增加。HMGB1作用后,RAW264.7细胞的NF-κB核转移明显增强,HSF-1表达明显增加(均P<0.05)。干扰HSF-1表达后,HMGB1诱导RAW264.7细胞产生的TNF-α量较未干扰的RAW264.7细胞明显增加(P<0.05)。结论:HMGB1能诱导RAW264.7细胞的炎症反应与HSF-1的表达,但HSF-1可能对HMGB1诱导的炎症反应有负反馈性抑制作用。


Role of HSF-1 in inflammatory response induced by HMGB1

Abstract

Objective: To investigate the role of heat shock transcription factor 1 (HSF1) in inflammatory response induced by high mobility group protein B1 (HMGB1). Methods: The TNF-α contents in the supernatants of RAW264.7 cells after exposure to different concentrations of HMGB1 for different times were determined by ELISA assay. The NF-κB nuclear translocation and HSF-1 expression in RAW264.7 cells after HMGB1 treatment were examined by immunofluorescence and Western blot, respectively. The change in TNF-α expression induced by HMGB1 in RAW264.7 cells after interference of HSF-1 expression was observed. Results: RAW264.7 cells presented two peaks of TNF-α release at 4 and 12 h respectively after HMGB1 treatment, and the TNF-α release level was increased with the elevation of HMGB1 concentration. The NF-κB nuclear translocation was significantly enhanced and HSF-1 expression was significantly increased in RAW264.7 cells after HMGB1 treatment (both P<0.05). The TNF-α expression induced by HMGB1 in RAW264.7 cells after interference of HSF-1 expression was significantly increased compared with RAW264.7 cells without HSF-1 interference (P<0.05). Conclusion: HMGB1 can induce inflammatory response and HSF-1 expression in RAW264.7 cells, but HSF-1 may play a negative feedback inhibitory role in the HMGB1-induced inflammation.


comments powered by Disqus

全文

引用

引用本文: 朝奇 尹, 全勇 贺, 成群 罗, 建大 周, 佳 陈, 萍 李, 颉 朱, 浩 彭, 阳成 徐, 如意 陶. HSF-1在HMGB1诱导的炎症反应中的作用 [J]. 中国普通外科杂志, 2016, 25(9): 1291-1295.
Cite this article as: YIN Chaoqi, HE Quanyong, LUO Chengqun, ZHOU Jianda, CHEN Jia, LI Ping, ZHU Jie, PENG Hao, XU Yangcheng, TAO Ruyi . Role of HSF-1 in inflammatory response induced by HMGB1 [J]. Chin J Gen Surg, 2016, 25(9): 1291-1295.