基础研究(Basic Research)

LncRNA SNHG15在甲状腺癌细胞中的表达及作用

Published at: 2016年第25卷第11期

帅勇锋 1 , 占大钱 2 , 王小军 1 , 张一中 1
1 宁波大学医学院附属医院 胃肠外科,浙江 宁波 315000
2 华中科技大学同济医学院附属同济医院 重症医学科,湖北 武汉 430000
通讯作者 一中 张 Email: shuaiyongfeng888@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.11.012
基金:

摘要

目的:探讨LncRNA SNHG15在甲状腺癌细胞中的表达及作用。方法:qRT-PCR检测人未分化甲状腺癌FRO细胞、人甲状腺鳞癌SW579细胞、人甲状腺导管癌TT细胞及正常甲状腺HT-ori3细胞中LncRNA SNHG15表达水平;将FRO细胞分别转染SNHG15 siRNA及阴性对照siRNA后,CCK8实验和克隆形成实验检测细胞增殖,流式细胞术检测细胞凋亡,Western Blot检测凋亡相关蛋白的表达。结果:LncRNA SNHG15在FRO细胞、SW579细胞和TT细胞中的表达量均明显高于正常甲状腺HT-ori3细胞,且在FRO细胞中表达量最高(均P<0.05);与转染阴性对照siRNA的FRO细胞比较,转染SNHG15 siRNA的FRO细胞增殖能力与克隆形成率均明显降低、细胞凋亡率明显增高、caspase-3与Bax蛋白表达量明显上调,而Bcl-2蛋白表达量明显下调(均P<0.05)。结论:LncRNA SNHG15在甲状腺癌细胞中表达升高,且细胞分化程度越低表达越高,沉默LncRNA SNHG15表达可抑制抑制甲状腺癌细胞增殖并促进凋亡。


Expression and action of LncRNA SNHG15 in thyroid cancer cells

Abstract

Objective: To investigate the expression and action of LncRNA SNHG15 in thyroid cancer cells. Methods: The LncRNA SNHG15 expressions in human undifferentiated thyroid carcinoma FRO cells, thyroid squamous cell carcinoma SW579 cells and human thyroid ductal carcinoma TT cells as well as normal thyroid HT-ori3 cells were determined by qRT-PCR. In FRO cells after transfection with SNHG15 siRNA or negative control siRNA sequences, the cell proliferation was tested by CCK8 assay and colony-forming assay, the cell apoptosis was measured by flow cytometry, and the expression levels of apoptosis-related protein were analyzed by Western blot, respectively. Results: The expression levels of LncRNA SNHG15 in FRO, SW579 and TT cells were all significantly higher than that in HT-ori3 cells, with the highest level in PRO cells (all P<0.05). In FRO cells transfected with SNHG15 siRNA compared with those transfected with negative control sequences, the proliferative and colony-forming abilities were significantly decreased, apoptosis rate was significantly increased, and the protein expressions of caspase-3 and Bax were up-regulated, while Bcl-2 protein expression was down-regulated significantly (all P<0.05). Conclusion: LncRNA SNHG15 expression is increased in thyroid cancer cells, and moreover, the poorer the differentiation of the cells, the higher is the expression of LncRNA SNHG15. LncRNA SNHG15 silencing can inhibit proliferation and promote apoptosis of thyroid cancer cells.


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引用

引用本文: 勇锋 帅, 大钱 占, 小军 王, 一中 张. LncRNA SNHG15在甲状腺癌细胞中的表达及作用 [J]. 中国普通外科杂志, 2016, 25(11): 1590-1595.
Cite this article as: SHUAI Yongfeng, ZHAN Daqian, WANG Xiaojun, ZHANG Yizhong . Expression and action of LncRNA SNHG15 in thyroid cancer cells[J]. Chin J Gen Surg, 2016, 25(11): 1590-1595.