基础研究(Basic Research)

Foxp3+调节性T细胞与乳腺癌淋巴结转移及增殖的关系

Published at: 2016年第25卷第11期

曾繁余 1 , 刘文慧 2 , 周心怡 2 , 张秋瑾 2 , 张杰 2 , 骆耐香 2
1 桂林医学院附属医院 乳腺甲状腺外科,广西 桂林 541001
2 桂林医学院 免疫学教研室,广西 桂林 541004
通讯作者 耐香 骆 Email: luonaixiang@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.11.014
基金:
国家自然科学基金资助项目 81260317
广西壮族自治区卫生厅基金资助项目 Z2013469
桂林市科学技术局科技攻关基金资助项目 20140120-1-10

摘要

目的:探讨Foxp3+调节性T细胞(Tregs)与乳腺癌淋巴结转移及增殖的关系。方法:用免疫组化法检测168例女性乳腺癌患者癌组织中CD4和Foxp3(调节性T细胞标志)的表达,以42例女性乳腺良性病变乳腺组织为对照,分析Tregs与乳腺癌淋巴结转移及乳腺癌组织细胞核增殖相关抗原Ki-67表达的关系。结果:乳腺癌组织中CD4+T细胞与Foxp3+T细胞(Tregs)的数量均高于乳腺良性病变组织,差异均有统计学意义(均P<0.05);有淋巴结转移的乳腺癌组织中CD4+T细胞与Foxp3+T细胞数均高于无淋巴结转移的乳腺癌组织,但仅后者差异有统计学意义(P<0.05)。乳腺癌组织中Tregs的浸润数量与Ki-67表达之间无关(P>0.05)。结论:乳腺癌组织微环境存在免疫抑制,Tregs浸润数量与淋巴结转移关系密切,但与肿瘤增殖无关,提示Tregs可作为判断乳腺癌患者有无淋巴结转移的新指标。


Relations of Foxp3+ regulatory T cells with lymph node metastasis and proliferation of breast cancer

Abstract

Objective: To investigate the relations of Foxp3+ regulatory T cells (Tregs) with lymph node metastasis and proliferation of breast cancer. Methods: The CD4 (marker of regulatory T cells) and Foxp3 expressions in breast cancer tissues from 168 female patients were determined by immunohistochemical staining, using tissue specimens from 42 female patients with benign breast disease as control. The relations of Tregs with lymph node metastasis of breast cancer and the expression of nuclear proliferation-associated antigen Ki-67 were analyzed. Results: The numbers of both CD4+ T cells and Foxp3+ T cells (Tregs) in breast tissue was higher than those in breast tissue with benign disease, and both differences had statistical significance (both P<0.05); the numbers of both CD4+ T cells and Foxp3+ T cells in breast tissue with lymph node metastasis were higher than those in breast tissue without lymph node metastasis, but only the latter had statistical significance (P<0.05). The number of infiltrating Tregs showed no significant association with Ki-67 expression in breast cancer tissue (P>0.05). Conclusion: There is immunosuppression in microenvironment of breast cancer tissue, and the number of infiltrating Tregs is closely associated with lymph node metastasis, but irrelevant to tumor proliferation. It suggests that Tregs can be used as a new indicator for estimating presence or absence of lymph node metastasis in breast cancer patients.


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引用

引用本文: 繁余 曾, 文慧 刘, 心怡 周, 秋瑾 张, 杰 张, 耐香 骆. Foxp3+调节性T细胞与乳腺癌淋巴结转移及增殖的关系[J]. 中国普通外科杂志, 2016, 25(11): 1602-1607.
Cite this article as: ZENG Fanyu, LIU Wenhui, ZHOU Xinyi, ZHANG Qiujin, ZHANG Jie, LUO Naixiang . Relations of Foxp3+ regulatory T cells with lymph node metastasis and proliferation of breast cancer [J]. Chin J Gen Surg, 2016, 25(11): 1602-1607.