基础研究(Basic Research)

犬下肢慢性深静脉血栓形成模型的构建

Published at: 2016年第25卷第12期

李林鹏 1 , 吴文皋 1 , 邓礼明 1 , 熊国祚 1 , 戴先鹏 1 , 申昕 1
1 南华大学附属第二医院 血管外科,湖南 衡阳 421001
通讯作者 礼明 邓 Email: nhxgwkdlm@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.12.011
基金:
湖南省教育厅青年基金资助项目 13B104

摘要

目的:建立犬慢性下肢深静脉血栓(DVT)模型。方法:9只成年杂种犬,任选一侧股静脉缩窄管腔面积80%~90%,以另一侧为对照,于缩窄处的远心端分支插入1根导管,注射凝血酶300 U,并阻断血流1 h,留置静脉导管作为静脉造影通路。观察犬术后下肢一般情况,比较造模前后凝血功能指标的变化,并行静脉顺行造影检查与病理学检查。结果:9只犬中有8只完成造模。犬造模后均出现跛行,实验侧下肢皮温升高;与术前比较,犬术后D-二聚体、纤维蛋白原凝含量较术前明显升高,凝血酶原时间明显降低,且以上指标以及实验侧与对照侧腿围差均在术后14 d达峰值,此后缓慢恢复(均P<0.05);术后28 d的静脉造影与病理学观察均证实慢性静脉血栓形成。结论:所建立的慢性DVT模型较贴合临床血栓形成病因及形成过程,且可持续行静脉造影检查,可用于DVT慢性期临床治疗及影像诊断方面研究。


Establishment of chronic lower limb deep venous thrombosis model in dogs

Abstract

Objective: To establish a canine model of chronic deep venous thrombosis (DVT) of the lower limbs. Methods: Nine mongrel dogs were used, in which, the femoral vein on one side was selected at random and narrowed down by 80% to 90% of the lumen area, and the femoral vein on contralateral side served as control; a catheter was inserted from the branch of the femoral vein at the distal end of the narrowing site, followed by 300 U thrombin injection and then underwent blood flow blockage for 1 h; the catheter was indwelled for venography pathway. The general conditions of lower limbs of the dogs after operation were observed, the parameters of coagulation function before and after operation were compared, and the prograde angiographic and pathological examinations were performed. Results: Model creation was successfully performed in 8 of the 9 dogs. After that, claudication and increased skin temperature of the affected lower limb were observed in all of them; the D-dimer and fibrinogen levels were significantly increased and the prothrombin time was significantly decreased in the dogs after operation compared with their values before operation, and all these variables along with the difference in leg circumferences between affected and control side reached their peak values on postoperative day (POD) 14, and then gradually alleviated (all P<0.05); the chronic venous thrombosis was verified by both venography and pathological examination on POD 28. Conclusion: The established chronic DVT model is relatively relevant to the cause and process of thrombosis in clinical setting, and also offers a continuous access to venography. So it can be used for studies on clinical treatment and imaging diagnosis of DVT.


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引用

引用本文: 林鹏 李, 文皋 吴, 礼明 邓, 国祚 熊, 先鹏 戴, 昕 申. 犬下肢慢性深静脉血栓形成模型的构建[J]. 中国普通外科杂志, 2016, 25(12): 1732-1737.
Cite this article as: LI Linpeng, WU Wengao, DENG Liming, XIONG Guozuo, DAI Xianpeng, SHEN Xin . Establishment of chronic lower limb deep venous thrombosis model in dogs [J]. Chin J Gen Surg, 2016, 25(12): 1732-1737.