基础研究(Basic Research)

环状RNA在肝细胞癌中的差异表达研究

Published at: 2017年第26卷第1期

高鹏骥 1 , 陈雷 1 , 高杰 1 , 李照 1 , 王福顺 1 , 冷希圣 1 , 朱继业 1
1 北京大学人民医院 肝胆外科/北京市肝硬化肝癌基础研究重点实验室,北京 100044
通讯作者 继业 朱 Email: gandanwk@vip.sina.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2017.01.011
基金:
北京大学人民医院研究与发展基金资助项目 RDC2015-01

摘要

目的:探讨环状RNA(circRNA)在肝细胞癌(HCC)与正常肝组织中表达谱的差异。方法:利用circRNA芯片技术检测3例HCC组织和癌旁肝组织circRNA的表达谱,经过对原始数据进行预处理、均一化后,找出差异表达的circRNA(与癌旁肝组织比较,HCC组织中1.5倍以上变化并且差异有统计学意义的circRNA定义为差异表达的circRNA)。根据表达差异倍数较高和样本间一致性较好的原则筛选circRNA,借助生物信息学软件预测可能受其调控的miRNA,结合文献检索初步确定可能在HCC中具有重要作用的circRNA。结果:与癌旁肝组织比较,HCC组织中的circRNA表达谱发生了明显变化。HCC组织中,1.5倍以上变化的circRNA共82条,其中上调21条,下调61条;5倍以上变化的circRNA共3条,其中上调2条,下调1条。最终筛选出在HCC组织中明显上调的hsa-circ-0043278(8.15倍,P=0.002)、hsa-circ-0006220(12.73倍,P=0.033)和明显下调的hsa-circ-0065214(6.28倍,P=0.019);生物信息学分析和文献检索显示,hsa-miR-520可能受hsa-circ-0043278和hsa-circ-0006220调控而影响HCC的发生和进展。结论:HCC组织circRNA表达谱发生了显著变化;hsa-circ-0043278和hsa-circ-0006220可能在HCC的发生和进展中发挥重要作用。


Differentially expressed circular RNAs in human hepatocellular carcinoma

Abstract

Objective: To analyze the difference in circular RNA (circRNA) expression profiles between hepatocellular carcinoma (HCC) tissue and normal liver tissue. Methods: The circRNA expression profiles in 3 paired specimens of HCC tissue and its adjacent liver tissue were detected by using the human circRNA microarray. After preprocessing and homogenization of the original data, the differentially expressed circRNAs were searched out (the circRNA in HCC tissue showed a greater than 1.5-fold change which was also statistically significant compared with adjacent liver tissue and was regarded as differentially expressed circRNA). Then, after these circRNAs screening according to the principle of higher fold change and better consistency and their putatively targeting miRNAs prediction by bioinformatics software combined with literature review, the circRNAs that potentially contribute to HCC development were preliminarily identified. Results: The circRNA expression profile in HCC showed remarkable changes compared with adjacent hepatic tissue. In HCC tissue, a total of 82 circRNAs showed a greater than 1.5-fold change, and of them, 21 were up-regulated and 61 were down-regulated; a total of 3 circRNAs showed a greater than 5-fold change, and of them, 2 were up-regulated and 1 was down-regulated. The significantly up-regulated circRNA hsa-circ-0043278 (8.15 fold, P=0.002) and hsa-circ-0006220 (12.73 fold, P=0.033), and significantly down-regulated circRNA hsa-circ-0065214 (6.28 fold, P=0.019) were finally obtained after screening. Bioinformatics analysis and literature review suggested that hsa-miR-520 was potentially regulated by hsa-circ-0043278 and hsa-circ-0006220, which contributed to the occurrence and development of HCC. Conclusion: The circRNA expression profile in HCC is greatly changed. Hsa-circ-0043278 and hsa-circ-0006220 may play important roles in the occurrence and development of HCC.


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引用

引用本文: 鹏骥 高, 雷 陈, 杰 高, 照 李, 福顺 王, 希圣 冷, 继业 朱. 环状RNA在肝细胞癌中的差异表达研究 [J]. 中国普通外科杂志, 2017, 26(1): 65-69.
Cite this article as: GAO Pengji, CHEN Lei, GAO Jie, LI Zhao, WANG Fushun, LENG Xisheng, ZHU Jiye . Differentially expressed circular RNAs in human hepatocellular carcinoma [J]. Chin J Gen Surg, 2017, 26(1): 65-69.