目的：构建G蛋白信号调节蛋白2（GPSM2）稳定高表达的胰腺癌细胞株，探讨GPSM2与人胰腺癌细胞迁移能力的关系。方法：构建GPSM2基因过表达质粒（pCMV-Tag 3B-GPSM2）并鉴定，将人胰腺癌MIA-PaCa-2细胞分别转染pCMV-Tag 3B-GPSM2（GPSM2转染组）或pCMV-Tag 3B空载体（阴性对照组），以无处理的MIA-PaCa-2细胞为空白对照，用RT-PCR检测各组细胞GPSM2 mRNA表达；Western blot检测各组细胞GPSM2、β-连环蛋白（β-catenin）的表达；用Transwell实验检测各组胰腺癌细胞迁移能力。结果：成功构建了GPSM2稳定高表达的重组细胞株。与空白对照组比较，GPSM2转染组细胞GPSM2 mRNA表达量明显上调，达前者73.3倍、GPSM2、β-catenin蛋白表达量明显升高、迁移细胞计数明显增加（均P<0.05）。此外，胰腺癌细胞中GPSM2与β-catenin的表达水平呈明显的正向线性关系（P<0.05）。阴性对照组与空白对照组间各指标的差异均无统计学意义（均P>0.05）。结论：上调胰腺癌细胞中GPSM2的表达能增加胰腺癌细胞的迁移能力，该作用可能与β-catenin蛋白表达升高有关。
Effects of GPSM2 over-expression on migration ability of human pancreatic cancer cells
Objective: To construct a stable pancreatic cancer cell line with high expression of the G-protein signaling modulator 2 (GPSM2), for investigating the relationship between GPSM2 and migration ability of human pancreatic cancer cells. Methods: The plasmids over-expressing GPSM2 gene (pCMV-Tag 3B-GPSM2) were constructed and then were identified. Human pancreatic cancer MIA-PaCa-2 cells were transfected with pCMV-Tag 3B-GPSM2 (GPSM2 transfection group) or empty pCMV-Tag 3B vectors (negative control group), with untreated MIA-PaCa-2 cells as blank control. In each group of cells, the GPSM2 mRNA expressions were measured by RT-PCR, the protein expressions of GPSM2 and β-catenin were determined by Western blot analysis, and the migration abilities were tested by Transwell assay, respectively. Results: The recombinant cell line with stable high GPSM2 expression was successfully constructed. In GPSM2 transfection group compared with blank control group, the GPSM2 mRNA expression was significantly up-regulated, with a 73.3-fold up-regulation, the protein expression levels of GPSM2 and β-catenin were significantly elevated, and the number of migrated cells was significantly increased (all P<0.05). In addition, a positive linear relationship existed between GPSM2 and β-catenin expressions in pancreatic cancer cells (P<0.05). There was no statistical difference in any of the indexes between negative control group and blank control group (all P>0.01). Conclusion: Up-regulating GPSM2 expression in pancreatic cancer cells can increase the migration ability of pancreatic cancer cells, which may be associated with increased β-catenin protein expression.