目的：探讨胃癌组织中EphA2与VEGF-C的表达及临床意义。方法：用免疫组化法检测82例胃癌组织及其癌旁组织中EphA2与VEGF-C蛋白的表达，以及淋巴管密度（LVD）；用real-time PCR检测20例胃癌组织及其癌旁组织中EphA2与VEGF-C mRNA的表达。分析EphA2与VEGF-C表达与患者临床病理特点及淋巴管生成的关系。结果：EphA2与VEGF-C在胃癌组织中的阳性表达率均明显高于癌旁正常组织（65.8% vs. 42.6%；71.9% vs. 39.0%，均P<0.05）。EphA2与VEGF-C的高表达与肿瘤浸润深度、淋巴结转移、TNM分期有关（均P<0.001）。EphA2蛋白与VEGF-C蛋白高表达的胃癌组织中LVD计数分别明显高于两者低表达胃癌组织（15.25±5.41 vs. 10.95±5.41，P=0.001；14.87±5.71 vs. 11.00±5.01，P=0.006）。胃癌组织中EphA2与VEGF-C蛋白以及mRNA表达均呈正相关（r=0.375，P=0.001；r=0.559，P=0.01）。结论：EphA2与VEGF-C在胃癌组织中均呈高表达，且可能共同促进淋巴管生成与胃癌淋巴结转移。
Expressions of EphA2 and VEGF-C in gastric cancer and their association with lymphangiogenesis
Objective: To investigate the EphA2 and VEGF-C expressions in gastric cancer and their clinical significance. Methods: The protein expressions of VEGF-C and EphA2 as well as the lymphatic vessel density (LVD) in 82 paired specimens of gastric cancer and adjacent gastric tissue were determined by immunohistochemical staining. The mRNA expressions of EphA2 and VEGF-C in 20 paired specimens of gastric cancer and adjacent gastric tissue were detected by real-time RT-PCR. The relations of EphA2 and VEGF-C expressions with the clinicopathologic characteristics of the patients and lymphangiogenesis were analyzed. Results: The positive expression rates of both EphA2 and VEGF-C in gastric cancer tissue were significantly higher than those in adjacent gastric tissue (65.8% vs. 42.6%; 71.9% vs. 39.0%, both P<0.05). The high expressions of EphA2 and VEGF-C were significantly associated with the depth of tumor invasion, lymph node metastasis and TNM stage (all P<0.001). The LVD count in gastric cancer tissue with high EphA2 or VEGF-C expression was significantly higher than that in gastric cancer tissue with low EphA2 or VEGF-C expression (15.25±5.41 vs. 10.95±5.41, P=0.001; 14.87±5.71 vs. 11.00±5.01, P=0.006). There was a significant correlation between EphA2 and VEGF-C in either protein or mRNA expression in gastric cancer tissue (r=0.375, P=0.001; r=0.559, P=0.01). Conclusion: Both EphA2 and VEGF-C expressions are increased in gastric cancer tissue, and they may jointly promote lymphangiogenesis and lymph node metastasis in gastric cancer.