基础研究(Basic Research)

肝细胞肝癌患者血清microRNAlet-7a水平的变化及意义

Published at: 2017年第26卷第7期

陈勇治 1 , 舒健 1 , 王俊 1 , 吕品 1 , 张红辉 1
1 湖南师范大学第一附属医院/ 湖南省人民医院 胆道外科,湖南 长沙 410005
通讯作者 俊 王 Email: 13907310355@139.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2017.07.012
基金:
湖南省长沙市科学技术局科技计划资助项目 K1203042-31

摘要

目的:探讨肝细胞肝癌(HCC)患者血清microRNAlet-7a(let-7a)水平的变化及其对HCC的诊断价值。方法:用qRT-PCR检测60例HCC患者及46例健康体检者血清let-7a的表达,分析血清let-7a水平与HCC的临床病理因素的关系,用受试者工作曲线(ROC)分析其对HCC的诊断效能。结果:HCC患者血清let-7a相对表达量明显低于健康对照组人群(0.538 vs. 1.571,P<0.05);HCC患者血清let-7a水平达与癌栓形成有关(P<0.05),与性别、年龄、HBV感染、肝硬化、肿瘤直径、肿瘤个数、淋巴结转移、TNM分期、淋巴结转移、病理分级、AFP水平均无关(均P>0.05);以0.529为let-7a诊断HCC的最佳临界值,其灵敏度为79%,特异度为71%,曲线下面积(AUC)为0.77(95% CI=0.624~0.839);联合检测血清let-7a与AFP,诊断HCC的灵敏度为83%,特异性为97%,AUC为0.92(95% CI=0.866~0.987)。结论:HCC患者血清let-7a水平降低,let-7a可能成为诊断HCC的新的分子标志物,联合AFP检测可提高对HCC的诊断的准确性。


Alteration of serum microRNAlet-7a level in patients with hepatocellular carcinoma and its significance

Abstract

Objective: To investigate the alteration of serum microRNAlet-7a (let-7a) level in patients with hepatocellular carcinoma (HCC) and its diagnostic value for HCC. Methods: The let-7a expressions in 60 HCC patients and 46 healthy subjects undergoing health maintenance examination were measured by qRT-PCR. And then the relations of serum let-7a level with clinicopathologic factors of HCC patients were analyzed and the diagnostic efficacy of let-7a for HCC was determined by using a receiver operating characteristic curve (ROC). Results: The relative expression level of serum let-7a in HCC patients was significantly lower than that in healthy population (0.538 vs. 1.571, P<0.05). The serum let-7a level was significantly associated with the formation of tumor emboli (P<0.05), but was irrelevant to all other studied factors that included sex, age, HBV infection, cirrhosis, tumor diameter, tumor number, lymph node metastasis, TNM stage, pathological grade and AFP level (all P>0.05). At an optimal cut-off value of 0.529 of let-7a for diagnosis of HCC, the sensitivity was 79%, the specificity was 71% and the area under the curve (AUC) was 0.77 (95% CI=0.624–0.839) respectively; in combined detection of serum let-7a and AFP for diagnosis of HCC, the sensitivity was 83%, the specificity was 97% and the AUC was 0.92 (95% CI=0.866–0.987), respectively. Conclusion: The serum let-7a level is decreased in HCC patients, which may be potentially used as a new molecular marker for diagnosis of HCC, and its diagnostic efficacy for HCC can be enhanced by combination detection of AFP.


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引用

引用本文: 勇治 陈, 健 舒, 俊 王, 品 吕, 红辉 张. 肝细胞肝癌患者血清microRNAlet-7a水平的变化及意义[J]. 中国普通外科杂志, 2017, 26(7): 889-894.
Cite this article as: CHEN Yongzhi, SHU Jian, WANG Jun, LU Pin, ZHANG Honghui . Alteration of serum microRNAlet-7a level in patients with hepatocellular carcinoma and its significance [J]. Chin J Gen Surg, 2017, 26(7): 889-894.