基础研究(Basic Research)

下调NDRG1 表达对胰腺癌细胞增殖与凋亡的影响

Published at: 2015年第24卷第3期

张小薄 1 , 高峰 1 , 谭晓冬 1 , 周磊 1 , 王怀涛 1 , 石刚 2
1 中国医科大学附属盛京医院 普通外科,辽宁 沈阳110004
2 辽宁省肿瘤医院 大肠外科,辽宁 沈阳 110042
通讯作者 晓冬 谭 Email: tanxd@sj-hospital.org
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.03.011
基金:
国家自然科学基金资助项目 30973501

摘要

目的:探讨NDRG1 在胰腺癌细胞中的表达及其与MMP-7 的关系。 方法: 检测NDRG1 与MMP-7 蛋白与mRNA 在4 种胰腺癌细胞株(PANC-1、BXPC-3、CAPAN-2、 SW1990)表达;构建靶向NDRG1 的干扰质粒siNDRG1,转染PANC-1 细胞,检测转染后PANC-1 细 胞NDRG1 与MMP-7 蛋白与mRNA 的表达,以及增殖与调亡情况。 结果:4 种细胞株中均有NDRG1 与MMP-7 蛋白与mRNA 的表达,且两者的表达水平随着细胞分化程 度的降低而升高(均P<0.05);PANC-1 细胞转染siNDRG1 后,NDRG1 与MMP-7 的蛋白与mRNA 表 达均明显降低、细胞增殖明显抑制、凋亡率明显升高(均P<0.05)。 结论:NDRG1 的表达与胰腺癌细胞的分化程度密切相关,且可能通过上调MMP-7 表达促进胰腺癌细 胞的生长。


Influence of down-regulation of NDRG1 expression on proliferation and apoptosis in pancreatic cancer cells

Abstract

Objective: To investigate NDRG1 expression and its relation with MMP-7 in pancreatic cancer cells. Methods: The protein and mRNA expressions of NDRG1 and MMP-7 in 4 different pancreatic cancer cell lines (PANC-1, BXPC-3, CAPAN-2 and SW1990) were determined. The interference plasmids siNDRG1 were transfected into PANC-1 cells after their construction, and then the protein and mRNA expressions of NDRG1 and MMP-7, cell proliferation and apoptosis after transfection were determined. Results: All the 4 cell lines presented either the protein or mRNA expressions of both NDRG1 and MMP-7, and their expression levels were increased with decrease of the degree of cell differentiation (all P<0.05). In PANC- 1 after siNDRG1 trasfection, the protein and mRNA expressions of both NDRG1 and MMP-7 were reduced, proliferation was suppressed, and apoptosis rate was increased significantly (all P<0.05). Conclusion: NDRG1 expression is closely related to the degree of differentiation of pancreatic cancer cells, and may probably promote cell growth through up-regulating MMP-7 expression.


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引用

引用本文: 小薄 张, 峰 高, 晓冬 谭, 磊 周, 怀涛 王, 刚 石. 下调NDRG1 表达对胰腺癌细胞增殖与凋亡的影响[J]. 中国普通外科杂志, 2015, 24(3): 363–369.
Cite this article as: ZHANG Xiaobao, GAO Feng, TAN Xiaodong, ZHOU Lei, WANG Huaitao, SHI Gang . Influence of down-regulation of NDRG1 expression on proliferation and apoptosis in pancreatic cancer cells [J]. Chin J Gen Surg, 2015, 24(3): 363–369.