基础研究(Basic Research)

Luminal A型和HER-2阳性乳腺癌的肿瘤干细胞生物学行为比较

Published at: 2017年第26卷第11期

董华英 1 , 王伟 1 , 陈元文 2 , 包俊杰 2 , 陈鑫 2 , 吴诚义 2
1 海南省人民医院 乳腺外科,海南 海口 570311
2 重庆医科大学附属第一医院 普通外科,重庆400016
通讯作者 诚义 吴 Email: NFMWK1192@hospital-cqmu.com
DOI: 10.3978/j.issn.1005-6947.2017.11.010
基金:
海南省卫生计生科教基金资助项目 02A2150014P1

摘要

目的:比较人Luminal A型和HER-2阳性乳腺癌的肿瘤干细胞的生物学行为差异。方法:用Luminal A型和HER-2阳性乳腺癌组织培分离并培养原代乳腺癌细胞,获取富含肿瘤干细胞的微球体(MS);用胰酶将MS消化成单细胞(微球体细胞,MSDC),比较两类乳腺癌MSDC的体外增殖和自我更新能力、ALDH1+表型肿瘤干细胞含量,以及移植NOD/SCID小鼠后的成瘤情况。结果:Luminal A型和HER-2阳性乳腺癌组织分离的原代乳腺癌细胞均能培养出MS,但相对于Luminal A型乳腺癌,HER-2阳性乳腺癌来源的MS形成时间短,生成的MS体积大;HER-2阳性乳腺癌来源的MSDC无论在无血清或添加血清的培养基中的增殖与再生能力均明显强于Luminal A型乳腺癌来源的MSDC,且前者含ALDH1表型干细胞比例明显高于后者(均P<0.05);HER-2阳性乳腺癌的MSDC在NOD/SCID小鼠体内成瘤能力也强于Luminal A型乳腺癌来源的MSDC。结论:Luminal A型和HER-2阳性乳腺癌分离出来肿瘤干细胞生物学行为存在明显的差异,两者可能有不同肿瘤干细胞起源。


Comparison of biological behaviors in cancer stem cells derived from Luminal A and HER-2-positive breast cancer

Abstract

Objective: To compare the difference in biological behaviors between two types of tumor stem cells derived from human Luminal A and HER-2-positive breast cancer. Methods: Primary breast cancer cells were isolated from specimens of Luminal A and HER-2-positive breast cancer tissues and cultured, and then the mammospheres (MSs) were obtained, which were highly enriched in tumor stem cells. MSs were dissociated into single cells (mammospheres-derived cells, MSDCs) by trypsin digestion, and then the proliferative and regenerative abilities in vitro, the proportions of ALDH1+ cell population, and the tumor formation abilities after inoculation into NOD/SCID mice were compared between MSDCs from the two types of breast cancer. Results: MSs formed in the primary cultured breast cancer cells either isolated from Luminal A or HER-2-positive breast cancer tissue. However, in HER-2-positive breast cancer, the MSs formation time was shorter and their volume was larger than those in Luminal A breast cancer. The proliferative and regenerative abilities in MSDCs from HER-2-positive breast cancer were significantly higher than those in MSDCs from Luminal A breast cancer either cultured in serum-free medium or medium containing serum, and the proportion of ALDH1+ cell population in the former was significantly higher than that in the latter (all P<0.05). The tumor formation ability of MSDCs from HER-2-positive breast cancer was stronger than that of MSDCs from Luminal A breast cancer in NOD/SCID mice. Conclusion: The tumor stem cells isolated from Luminal A and HER-2-positive breast cancer have significantly different biological behaviors. So the two types of breast cancer may originate from different tumor stem cells.


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引用

引用本文: 华英 董, 伟 王, 元文 陈, 俊杰 包, 鑫 陈, 诚义 吴. Luminal A型和HER-2阳性乳腺癌的肿瘤干细胞生物学行为比较[J]. 中国普通外科杂志, 2017, 26(11): 1431-1438.
Cite this article as: DONG Huaying, WANG Wei, CHEN Yuanwen, BAO Junjie, CHEN Xin, WU Chengyi . Comparison of biological behaviors in cancer stem cells derived from Luminal A and HER-2-positive breast cancer[J]. Chin J Gen Surg, 2017, 26(11): 1431-1438.