目的：探讨表皮生长因子受体ErbB4在人肝内胆管癌（ICC）组织中的表达及意义。方法：采用免疫组化法分别检测24例ICC患者癌组织（ICC组）、癌旁胆管组织（癌旁组）及16例肝内胆管结石患者胆管组织（结石组）中ErbB4的表达，并分析ErbB4表达与ICC 患者临床病理因素的关系。结果：ErbB4 的强阳性表达率在ICC组中为75.0%，明显高于癌旁组（45.8%）和结石组（37.5%）（均P<0.05）；且ICC组胞核ErbB4阳性表达率高于另两组（均P<0.05），而胞膜与胞质ErbB4阳性表达率3组间差异无统计学意义（均P>0.05）。ErbB4的高表达与ICC患者淋巴结转移及TNM 分期有关（均P<0.05）。结论：ErbB4 的表达增高与核内移与ICC 的发生发展以及浸润转移密切相关。
ErbB4 expression in intrahepatic cholangiocarcinoma and its clinical significance
Objective: To investigate the expression of epidermal growth factor receptor ErbB4 in human intrahepatic cholangiocarcinoma (ICC) tissues and its significance. Methods: The ErbB4 expressions in 24 paired specimens of ICC tissue (ICC group) and adjacent bile duct tissue (tumor adjacent group), and 16 specimens of bile duct tissue from hepatolithiasis patients (hepatolithiasis group) were detected by immunohistochemical staining, and the relations of ErbB4 expression with the clinicopathologic factors of ICC patients were also analyzed. Results: The strong positive expression rate of ErbB4 in ICC group was 75.0%, which was significantly higher than that in tumor adjacent group (45.8%) or hepatolithiasis group (37.5%) (both P<0.05), nuclear expression rate of ErbB4 in ICC group was significantly higher than that in the other two groups (both P<0.05), while the membranous and cytoplasmic expression rates of ErbB4 showed no significant difference among the three groups (both P>0.05). High ErbB4 expression was significantly associated with lymph node metastasis and TNM stage of ICC patients (both P<0.05). Conclusion: Increased expression of ErbB4 and its transfer into cellular nucleus are closely related to the occurrence and development as well as infiltration and metastasis of ICC.