目的：探讨基质金属蛋白酶2（MMP-2）与基质金属蛋白酶9（MMP-9）在肝内胆管细胞癌（ICC）组织中的表达及其与ICC 患者临床病理特征及预后的关系。方法：收集2011 年1 月—2014 年12 月收治的50 例ICC 患者的癌组织与癌旁组织手术标本，采用免疫组织化学方法及RT-PCR 方法检测MMP-2 和MMP-9 在以上组织中的表达，分析MMP-2 和MMP-9表达与ICC 患者临床病理参数以及术后生存率的关系。结果： 免疫组织化学结果显示，ICC 组织中MMP-2 和MMP-9 蛋白阳性表达率分别为34.0% 和32.0%，而两者在癌旁组织中无阳性表达；RT-PCR 结果显示，ICC 组织中MMP-2 和MMP-9 的相对表达均明显高于癌旁组织（均P<0.05）。MMP-2 和MMP-9 表达与ICC 患者是否存在淋巴结转移和肿瘤分化程度有关（均P<0.05），而与患者性别、年龄和肿瘤分期无关（均P>0.05）；MMP-2 和MMP-9阳性患者生存率明显低于各自阴性者，而两者均阳性患者的生存率最低（均P<0.05）。结论：ICC 组织中MMP-2 和MMP-9 表达明显上调，且两者的表达与ICC 患者恶性临床病理特征及不良预后密切相关。
MMP-2 and MMP-9 expressions in intrahepatic cholangiocarcinoma tissue and the significance
Objective: To investigate the expression of matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) in intrahepatic cholangiocarcinoma (ICC) and their relations with the clinicopathologic features and prognosis of ICC patients. Methods: The surgical specimens including tumor and tumor-adjacent tissue from 50 ICC patients treated between January 2011 and December 2014 were collected. The MMP-2 and MMP-9 expressions in these tissues were detected by immunohistochemical staining and RT-PCR method, and the relations of MMP-2 and MMP-9 expressions with clinicopathologic variables and postoperative survival of ICC patients were analyzed. Results: Results of immunohistochemical staining showed that the positive expression rate of MMP-2 and MMP- 9 in ICC tissue was 34.0% and 32.0% respectively, but neither was positive in tumor-adjacent tissue; results of RTPCR showed that both relative expression levels of MMP-2 and MMP-9 in ICC tissue were significantly higher than those in tumor-adjacent tissue (both P<0.05). Both MMP-2 and MMP-9 expressions were significantly associated with the presence or absence of lymph node metastasis and degree of tumor differentiation of ICC patients (all P<0.05), but were irrelevant to the gender, age and tumor stage of ICC patients (all P>0.05); the survival rate in patients with positive MMP-2 or MMP-9 expression was significantly lower than that in patients with negative MMP-2 or MMP-9 expression, and those with both MMP-2 and MMP-9 positive expressions had lowest survival rate (all P<0.05). Conclusion: MMP-2 and MMP-9 expressions are increased in ICC tissue, and both expressions are closely related to the malignant clinicopathologic profiles and poor prognosis of ICC patients.